CD117/c-kit Antibody AF1356 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD117/c-kit Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF1356
NCBI Gene AliasesPBT, Fdc, MASTC, C-Kit, Gsfsow3, SOW3, W, CD117, SCFR, Ssm, SCO5, SCO1, Bs, Gsfsco1, Tr-kit, Gsfsco5, c-KIT
IsotypeIgG
ApplicationsWestern Blot, Simple Western, Flow Cytometry, Immunohistochemistry, CyTOF-ready
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetCD117/c-kit
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityHuman, Mouse
NCBI Full Gene NameKIT proto-oncogene receptor tyrosine kinase, KIT proto-oncogene, receptor tyrosine kinase
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit, Gln25-Thr519 (Ala207Glu), Accession # P05532
ConcentrationLYOPH
Brand/TrademarkR&D Systems, a Bio-Techne Brand
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human and mouse CD117/c-kit in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Core binding factor subunit β plays diverse and essential roles in the male germlineFrontiers in Cell and Developmental BiologyNovember 2, 2023Mustika Rahmawati, Kassie M. Stadler, Blanca Lopez-Biladeau, Tia M. Hoisington, Nathan C. LawLIN28A,"R&D Systems, AF3757","IF, NanoString",IF 1:250 NanoString 1:125 Goat anti cKIT,"R&D Systems, AF1356","IF, IHC",1:125 Rabbit anti STRA8,"Michael Griswold, WSU",IHC,1:200 Rat anti TRA98,"Abcam, ab82527",IF,1:500 Rabbit

(2) Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysisNature Cell BiologyOctober 5, 2023Takahito Iga, Hiroshi Kobayashi, Dai Kusumoto, Tsukasa Sanosaka, Nobuyuki Fujita, Ikue Tai-Nagara, Tomofumi Ando, Tomoko Takahashi, Koichi Matsuo, Katsuto Hozumi, et al.(GFP)-Alexa Fluor-488 (Molecular Probes, catalogue no. A21311, 1:500), anti-c-Kit (R&D Systems, catalogue no. AF1356, 1:500), anti-Sp7/Osx (Abcam, catalogue no. ab22552, 1:500), anti-cathepsin K (Abcam, catalogue no.

(3) WNK1 is required during male pachynema to sustain fertilityiScienceSeptember 15, 2023Ru-pin Alicia Chi, Xiaojiang Xu, Jian-Liang Li, Xin Xu, Guang Hu, Paula Brown, Cynthia Willson, Oleksandr Kirsanov, Christopher Geyer, Chou-Long Huang, et al.rabbit monoclonal anti-WNK1,Abcam,Cat#ab174854No RRID Goat polyclonal anti-c-KIT,R and D Systems,Cat# AF1356; RRID: AB_354750 Rat monoclonal anti-GCNA1 (TRA98),Abcam,Cat# ab82527; RRID: AB_1659152 Alexa Fluor®

(4) RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosisNucleic acids researchAugust 11, 2023Liu C, Wang L, Li Y, Guo M, Hu J, Wang T, Li M, Yang Z, Lin R, Xu W, et al.(11802-1-AP), HOP2 (11339-1-AP) were purchased from Proteintech (Rosemont, USA). Goat antibody to c-KIT (AF1356) was purchased from R&D Systems (Minneapolis, USA). Rabbit antibodies to Tubulin (AC007) and GAPDH (AC001)

(5) Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cellsCell reportsJuly 25, 2023Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford PA, Zielen AC, Orwig KE, Wang N.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies PLZF,Santa Cruz,Cat# sc-28319; RRID:AB_2218941 KIT,R&D Systems,Cat# AF1356; RRID:AB_354750 SLC7A5,Cell Signaling Technology,Cat# 5347S; RRID:AB_10695104 SLC38A2,Sigma Aldrich,Cat#

(6) An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fateScience advancesJuly 14, 2023Hansen SL, Larsen HL, Pikkupeura LM, Maciag G, Guiu J, Müller I, Clement DL, Mueller C, Johansen JV, Helin K, et al.A-11122; RRID: AB_221569), anti-KIT(1:200 dilution; polyclonal goat antibody; R&D Systems, catalog no. AF1356; RRID: AB_354750), anti-LYVE1 (1:300 dilution; polyclonal goat antibody; R&D Systems, catalog no. AF2125;

(7) Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesiseLifeJune 2, 2023Nakajima-Takagi Y, Oshima M, Takano J, Koide S, Itokawa N, Uemura S, Yamashita M, Andoh S, Aoyama K, Isshiki Y, et al.RRID:AB_823447,IF (1:100) Antibody,Human/mouse CD117/c-kit antibody (goat polyclonal),R&D Systems,Cat# AF1356; RRID:AB_354750,IF (1:100) Antibody,"CD41a monoclonal antibody (eBioMWReg30 (MWReg30)), Biotin (rat

(8) Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removalCell stem cellMay 4, 2023Hao X, Shen Y, Chen N, Zhang W, Valverde E, Wu L, Chan HL, Xu Z, Yu L, Gao Y, et al.

(9) Sertoli cells are the unique source of stem cell factor for spermatogenesisDevelopmentMarch 15, 2023Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhouanti-SMA (1:250; Abcam, ab5694), anti-CD31 (1:250; R&D Systems, AF3628), anti-c-Kit (1:100; R&D Systems, AF1356), anti-GFP (1:400; Aves Labs, GFP-1020), anti-PLZF (1:200; Santa Cruz Biotechnology, sc-28319), anti-STRA8

(10) Insulin-Like Growth Factor1 Preserves Gastric Pacemaker Cells and Motor Function in Aging via ERK1/2 ActivationCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Truong Thuy Nguyen V, Taheri N, Choi EL, Kellogg TA, Linden DR, Hayashi Y.pAb,Ab81086,IgG/GR12174-15,,0.5 μg/mL IGF1,CST,Rabbit mAb,73034,IgG,,1:2000 KIT,R&D Systems,Goat pAb,AF1356,IgG/IEO0217101,,0.2 μg/mL for mice KIT,"DAKO (Carpinteria, CA)",Rabbit pAb,A4502,100428020A,,1:4000

(11) SRSF1-mediated alternative splicing is required for spermatogenesisInternational journal of biological sciencesJanuary 1, 2023Lei WL, Li YY, Du Z, Su R, Meng TG, Ning Y, Hou G, Schatten H, Wang ZB, Han Z, et al.AF2944, R&D Systems); SFRS1 polyclonal antibody (rabbit, 12929-2-AP, Proteintech); c-Kit antibody (goat, AF1356, R&D Systems). Horseradish peroxidase-conjugated secondary antibodies were purchased from Zhongshan

(12) SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouseNature communicationsDecember 21, 2022Uchida A, Imaimatsu K, Suzuki H, Han X, Ushioda H, Uemura M, Imura-Kishi K, Hiramatsu R, Takase HM, Hirate Y, et al.monoclonal anti-CDH1 (BD Transduction lab, 610181, 1:400), goad polyclonal anti-c-KIT (R&D systems, AF1356, 1:200), rabbit monoclonal anti-EPCAM (Abcam, ab32392, 1:100), goat polyclonal anti-GATA4 (Santa Cruz,

(13) New C3H Kit(N824K/WT) cancer mouse model develops late-onset malignant mammary tumors with high penetranceScientific reportsNovember 17, 2022Klein-Rodewald T, Micklich K, Sanz-Moreno A, Tost M, Calzada-Wack J, Adler T, Klaften M, Sabrautzki S, Aigner B, Kraiger M, et al.anti-B220 (catalog no.550286, 1:50, BD PharMingen, California, USA), anti-KIT antibody (catalog no. AF1356, 1:40, R&D Systems,Minnesota, USA), anti-CD3 (catalog no. ZYT-RBG024, 1:8, diagnostic-Biosystems, California,

(14) Modeling mammalian spermatogonial differentiation and meiotic initiation in vitroDevelopmentNovember 15, 2022Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick, P. Hande Ozdinler, Douglas A. Gray, Kelsey Fisher-Wellman, Brian P. Hermann, et al.Abcam, rat monoclonal), anti-GFRA1 (1:800, AF560, R&D Systems, goat polyclonal), anti-KIT (1:1000, AF1356, R&D Systems, goat polyclonal), anti-ZBTB16 (1:2000, ab189849, Abcam, rabbit polyclonal), anti-UCHL1

(15) Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysisCell stem cellNovember 3, 2022Zheng Z, He H, Tang XT, Zhang H, Gou F, Yang H, Cao J, Shi S, Yang Z, Sun G, et al.

(16) The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in micePLoS geneticsSeptember 1, 2022Peart NJ, Johnson TA, Lee S, Sears MJ, Yang F, Quesnel-Vallières M, Feng H, Recinos Y, Barash Y, Zhang C, et al.number TRA98,rat,Abcam,1:1000,ab82527 ZBTB16/PLZF,Goat,R & D Systems,1:1000,AF2944 KIT,Goat,R & D Systems,1:1000 (IIF); 1:500 (IHC),AF1356 SYCP3-488,Mouse,Abcam,1:200,Ab205846 FLAG,Rat,Novus,1:250,NBP1-06712SS STRA8,Rabbit,Abcam,1:3000,ab49602 GATA4,Rabbit,Cell Signaling Technology

(17) Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor CellsFrontiers in Bioengineering and BiotechnologyAugust 4, 2022Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttualaimmunostaining with CD31 (1:200, Abcam, ab28364), CD34 (1:200, Bio-Rad, MCA 1825), and CD117 (R&D systems, AF1356) antibodies or alternatively with VEGFR2 (1:200, Santa Cruz Biotechnology, sc-393163) antibody. Alexa

(18) The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure OutcomesFront Endocrinol (Lausanne)May 31, 2022Sarah C. Moody, Penny A. F. Whiley, Patrick S. Western, Kate L. Lovelandgoat; 1:400), VASA (Cell Signalling Technologies; 8761S; raised in rabbit; 1:400), cKIT (R&D Systems; AF1356; 1:100), Laminin (Sigma; L9393; 1:200) and AMH (Santa Cruz; sc6886; 1:200). Slides were incubated overnight

(19) Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesisCommunications biologyMay 26, 2022Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro KI, Ogawa T, Yoshida S.polyclonal antibody (R&D Systems, #AF560, 1:1000); anti-KIT goat monoclonal antibody (R&D Systems, #AF1356, 1:1000), anti-mouse Vasa homolog (MVH) rabbit polyclonal antibody (Abcam, #ab13840, 1:500); anti-SCP3

(20) Stimulation of C-Kit+ Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal DegenerationFrontiers in PharmacologyMarch 31, 2022Xi Chen, Shanshan Li, Xiaoli Liu, Jingjie Zhao, Lanting Wu, Ran You, Yanling Wang2016; Zou et al., 2019). The primary antibodies used were as follows: anti-c-kit at 10 μg/ml (Cat# AF1356, R&D Systems, United States), anti-glutamine synthetase (GS) at 1:200 (Cat# ab73593, ab64613; Abcam,

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(1)

3/1/2022Good CD117 Antibody for Immunostaining James CarrollStem cell factor receptor (CD117) and its ligand (also named c-kit ligand) play essential roles in gametogenesis, melanogenesis and hematopoiesis.

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CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (109)

Reviews

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Webinar: Improving Cancer Biomarker Detection with Multiplex Technology

Screening of Leukemic Neoantigen Specific T cells with the iQue Screener PLUS

CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

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